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Biotechnology as the name indicates, includes Biological organisms and technology. The use of technology to harvest the resources from biological organisms might involve modification or upgrade of the living systems of the organism. Biotechnology might also copy the living reactions at an industrial level.
The resources can be applied to the welfare of humans in terms of economy, and health. Majorly speaking, biotechnology is the exploitation of services by microbes at a large scale or manufacturing level to produce a product.
Biotechnology has its roots from time immemorial, from the age of early man. In a true sense, from then on, the civilisation of human beings started, not only that, but he also started primitive biotechnology. This primitive biotechnology, based on observation, trial and error, which was too slow, involved crossbreeding to produce better yields, which made his life better.
Fermentation in the food industry is another application of Integrated Science, that involves Microbiology and Biotechnology. Humans used the fermentation reactions in microbes to modify the properties of food.
The modern phase of biotechnology started in the year 1973, with the birth of genetic engineering technology. This key event has split biotechnology into two.
Gene Biotechnology: This involves the manipulation of genes – Genetic Engineering and cloning.
Non-Gene Biotechnology: This involves whole cells and tissues, which may or may not be altered by Genetic engineering and cloning, involving tissue culture, microbial fermentation, hybrid production, and hybridoma production that involves Monoclonal antibodies (MABs).
Key Events in the Development of Biotechnology:
History in the development of Biotechnology as an applied science can be studied in two different phases. The years before 1973 and the years after 1973.
The years before 1973 involved classical biotechnology, which marked the increasing knowledge and scientific approaches to understanding the life processes and meagre applications that lacked genetic innovations.
Years after 1973 involved genomic manipulations, genomic data explosion and parallel development in computing and machine engineering, this heralded the modern phase of biotechnology.
Ancient Biotechnology (Pre–1800)
Years | Contribution |
7000 BC | Beer preparation form honey, rice – (Sumerians and Babylonians) |
Till 800 | Fermentation and Domestication of Plants |
Classical Biotechnology (1800-1950)
Scientific evidence for the phenomenon of Biotechnology and increasing knowledge in the areas of biology.
Modern Biotechnology (1950-Present)
The Era of Genetic Biotechnology
1953 – DNA helix model
1961 – Central Dogma
1961 – Operon Concept, gene interaction understanding
1966 – Genetic Code Cracked
1973 – Exonucleases and endonucleases (Genetic Engineering and the birth of rDNA technology)
1975 – Hybridoma Technology
1977 – Synthesising of human insulin in E. coli using genetic engineering
1990 – Gene Therapy
1994 – First approved Genetically Modified Organisms GMOs).
1997 – Cloning
2000 – 2019 – Genetic sequencing, Cellular and tissue engineering.
2015 – Gene Editing Like CRISPR
2019 – Prime Editing (Superior to CRISPR)
Artificial Organ synthesis, Gene edits, and the development of breeds from Genomics are the present ongoing research in Biotechnology.
The following are the key areas, where Biotechnology has its impact:
Environment: Biodegradable products, bioremediation processes using GMOs, wastewater treatment using microbes, and catalytic properties of microbes, fungi, and plants are some of the friendly applications of biotechnology towards the environment.
Agriculture and Transgenics: Development of GMOs, which are tolerant to stresses, with superior yields and highly fortified. Culturing of microbes in large tanks to enhance the quality of food. GMOs can be made as the hubs to harvest biological Products.
Medicine: Vaccines, synthetic human hormones, Gene therapy, powerful diagnostics (ELISA, PCR, etc,.) to identify diseases, and synthetic organs are all the fruits of Genetic engineering and Cloning.
Biotechnology Assignment Help provides a foundation to help Australian students to help understand the concepts of Biotechnology, which span the timeline from past to present research.
Biotechnology Assignment Help offers great help in understanding the concepts of Biotechnology. Not only that we also aid Australians with the biological sciences that have some par role in the development of Biotechnology.
Botany: Botany is plant science. It includes the study of all the features of plants. The features include structure (Morphology and Anatomy), function (Physiology), evolutionary relations and classification. For more details, students can refer to our botany assignment help.
Zoology: Zoology is animal science. It includes the study of all the features of Animals. The features include structure (Morphology and Anatomy), function (Physiology), evolutionary relations and classification. Our experts provide the best zoology assignment help.
Microbiology: Study of microbiota. This study encompasses structure, functions, evolutionary relations, pathogenicity, and applications to human welfare. For in-depth information, refer to our microbiology assignment help from our top experts.
Genomics: Genomics is the science of genes. This science deals with the structure, functions, interactions, synthesis, sequencing, evolutionary aspects, and mapping of genes. Get Genomics Assignment Help for scoring top grades.
Biotechnology has got many definitions. Different Governments and Organisations of different countries have their definitions of Biotechnology .Looking at the definition of UK, it is clear that biotechnology is an applied science. With the knowledge obtained from the various fields of Biology, Biochemistry, Microbiology, and chemical engineering we obtain valuable goods, that raise the economic, and health status of mankind.
Biotechnology aims at sustainable development. Be it economics or health status, sustainable development must meet the needs of the present. It should not compromise the ability of future generations to meet their own needs.
There are four major branches of Biotechnology, each of which has a characteristic colour code.
Branch |
Definition |
Tools |
Applications as |
Marine Biotechnology (Blue) |
Utilisation of Marine and aquatic life |
Proteins, enzymes |
Antioxidants, antibiotics, analgesics, anti- fungal agents |
Medical Biotechnology (Red) |
Involves fighting diseases by using the biological traits of other beings |
Vaccines, antibiotics, and new drugs |
Therapeutic Compounds |
Industrial Biotechnology (White) |
Applications that involve industrial processes. |
Enzymes and eco-friendly processes. |
Produce valuable chemicals or chemicals that counteract the hazardous effect. |
Agricultural Biotechnology (Green) |
Application of biotechnology to reduce the dependence of agriculture on mechanical and chemical innovations |
Biological organisms and their products |
Reduce environmental damage and food security. |
The above said branches rely much on the following tabulated technologies,
Technology | Description |
Genetic Engineering | Manipulation of Organism genes to make a better product/protein. |
Tissue Culture | Artificial culture of cells/tissues in bulk in an artificial environment. |
Cloning | It involves, Gene Cloning: Making copies of genes. Reproductive Cloning: Creation of whole animals or plants. Therapeutic Cloning: Copies of stem cells. |
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Genetic Engineering: Genetic engineering is the technology that aims at altering the DNA makeup of an organism.
The basic and sequential steps involved in Genetic Engineering include:
Isolation of DNA from a donor organism
Insertion of Isolated DNA into a plasmid vector
Introduction and growth of recombinant vector (DNA of interest + Plasmid vector) into an appropriate host.
The tools and techniques that play a crucial role in this technology are
Enzymes
Cloning Vectors
Gene Transfer and Cloning
Screening of recombinants
Analysis of DNA.
Enzymes: The crucial enzymes involved in this technology include
Restriction endonucleases (They have the ability to make cuts in the DNA at appropriate locations termed as Palindromic sites)
Ligase (Ligases joins the DNA fragments. They seal the cuts generated by Restriction endonucleases).
Reverse Transcriptase (Converts RNA to DNA. Used in eukaryote gene expression studies).
Cloning Vectors: Vectors are carriers. They carry the genes generated by restriction endonucleases.
Vectors used in genetic engineering are Plasmids. But there can be many, such as bacteriophages, Cosmid, and Artificial chromosomes.
Plasmids are also cut by the restriction endonucleases. Generally, the restriction enzymes that cut the Gene of Interest and Vectors are the same. This step will ensure the generation of the same cuts which are complementary to each other and also easy joining by Ligases.
Ligation of Gene of Interest with Plasmid generates a Recombinant DNA.
Gene Transfer and Cloning:The recombinant DNA molecules can be transferred into a suitable host. There are many methods of Gene Transfer. They include
Transformation (We force the host cells to take up the DNA)
Electroporation (We use high voltage pulses to incorporate the DNA into a suitable host)
Liposome-Mediated Gene transfer (DNA molecules are delivered enclosed in lipid droplets)
Transduction (DNA molecules to be inserted into the organism are packed in disarmed plant and animal viruses
Biolistic (DNA coated with gold particle fragments are bombarded into the host cells)
Screening of Recombinants:The culture can include cells that have taken up the DNA, and cells that are devoid of DNA. There are 2 crucial steps in the selection of colonies.
Selection of Transformants from Non-transformants. Non-transformants do not have any genes inserted in them.
Selection of Recombinants from Non-recombinants in the Transformants.Non-recombinants are those cells with only plasmid vectors.Recombinants are those cells with recombinants vectors (Plasmid vectors with inserted gene)
Analysis of DNA :This is a crucial step in selecting the right choice of recombinants.
Recombinants have recombinant DNA molecules inserted into them. This Recombinant DNA should be analysed after isolating the DNA from recombinants.
Recombinants can be positive recombinants (Vector with GOI oriented in right orientation) and Negative recombinants (Vector with GOI oriented in wrong orientation) .
Agarose Gel electrophoresis is the technique that helps identify the Vector with the correct orientation.We need restriction enzymes to evaluate the positive and negative recombinants. Once we differentiate positive and negative colonies, we encourage the growth of Positive clones to obtain the right product at a large scale.
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Fermentation and how is it different from Aerobic and Anaerobic Respiration:The products of Fermentation are Organic Acids, Ethanol and Carbon dioxide. Organic acids and Ethanol have preservative effects. They limit the growth of pathogenic microbes and prevent the spoilage of food.Production of Organic Acids or ethanol requires no specialised organelles. Fermentation takes place in the cytoplasm. Fermentation is just the extension of Glycolysis and is different from Anaerobic Respiration.
The following table differentiates among Aerobic, anaerobic and Fermentation living reactions.
Aerobic Respiration |
Fermentation |
Anaerobic Respiration |
The very first step is Glycolysis |
The very first step is Glycolysis |
The very step is Glycolysis. |
Pyruvate is directed to Citric acid cycle. |
Pyruvate is directed to a two-step process. |
Pyruvate is directed to other complex pathways. |
Occurs in Cytoplasm and Mitochondria, |
Occurs in cytoplasm |
Occurs inn Cytoplasm. |
The energy compounds of Respiration, NADH are cycled through Electron transport chain (ETC). |
No ETC. NADH produced in Glycolysis is used to break down pyruvate to Organic acids, ethanol, and Carbon dioxide. |
NADH are recycled to ETC. |
38 ATP are released in this process |
Only 2 molecules of ATP are released. |
ATPs released are less than in aerobic and more than in Fermentation. |
Types of Fermentation : Fermentation is of two types. They include:
Alcoholic Fermentation
Acidic Fermentation
Alcoholic Fermentation: It is a three-step Reaction:
Glycolysis
Pyruvate decarboxylase (Removal of Carbon dioxide)
Alcohol dehydrogenase reaction (Generation of Ethanol)
Seen in especially in Yeasts. Pyruvate is Converted to Ethanol and Carbon dioxide. This conversion Regenerates NAD+ for Glycolysis.
Acidic Fermentation:Acidic Fermentation occurs in Bacteria. There are three types of Acidic Fermentation.
Lactic Acid fermentation
Acetic Acid fermentation
Butyric Acid fermentation
Lactic Acid fermentation: It involves two steps.
Step 1: Glycolysis
Step 2: Anaerobic fermentation (Conversion of Pyruvate to lactate).
Acetic Acid fermentation: Acetic acid formation involves three steps.
Ethanol fermentation by yeasts.
Step 2 and 3 happens in bacteria. E.g.: Acetobacter
Conversion of ethanol by acetaldehyde
Acetaldehyde dehydrogenation (Conversion of acetaldehyde to Acetic acid).
Butyric Acid Fermentation: The end product is Butyric acid. This happens in the bacteria Clostridium butylicum.
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